MEDIA COMPOSITIONS FOR MYCOPLASMA Russian patent published in 2024 - IPC C12N1/20 A61K39/02 

FIELD: biotechnology.

SUBSTANCE: disclosed is a Mycoplasma hyopneumoniae growth additive composition containing choline chloride, niacinamide, nicotinic acid, L-methionine, L-cysteine, putrescine dihydrochloride, thiamine pyrophosphate, sodium L-ascorbate, spermine, pyridoxal-5'-phosphate monohydrate, tetrahydrofolic acid, 3'-dephosphocoenzyme and riboflavin in a given amount; its use in the manufacture of a medicinal product for preventing, relieving or improving the condition in human or other animal diseases caused by Mycoplasma hyopneumoniae; method for culturing Mycoplasma hyopneumoniae in a medium containing a basic nutrient medium selected from a group consisting of Frey's medium and a medium with porcine cerebral heart extract, horse serum and Mycoplasma hyopneumoniae growth supplement; method of producing an immunogenic composition, comprising culturing Mycoplasma hyopneumoniae in a medium containing a basic nutrient medium selected from a group consisting of Frey's medium and a medium with porcine cerebral heart extract, horse serum and Mycoplasma hyopneumoniae growth additive, Mycoplasma hyopneumoniae incubation at 37°C; and Mycoplasma hyopneumoniae inactivation.

EFFECT: inventions provide Mycoplasma growth with preservation of immunogenicity.

10 cl, 4 dwg, 15 tbl, 8 ex