METHOD FOR SIMULATING ASPHYXIAL CIRCULATORY ARREST AND POSTRESUSCITATION DISEASE Russian patent published in 2024 - IPC G09B23/28 A61B5/02 

FIELD: experimental medicine.

SUBSTANCE: invention can be used to study the pathogenesis of postresuscitation disease after asphyxiation, as well as for assessing safety and effectiveness of new methods of treating postresuscitation disease. General anesthesia is performed by intraperitoneal introduction of a combination of anesthetics, which is maintained until the end of the complex of intensive care measures in the postresuscitation period. For fixation of circulatory arrest, as well as for diagnosis of spontaneous circulation recovery after resuscitation by monitoring the ECG and blood pressure, the laboratory animal’s extremity skin perfusion is additionally evaluated using laser Doppler flowmetry (LDF). Duration of circulatory arrest is recorded from moment of electromechanical dissociation of heart, action of combined general anesthesia is continued during induction of asphyxia, circulatory arrest and in early postresuscitation period.

EFFECT: method enables to specify the moment of arrest and recovery of spontaneous blood circulation in rats under conditions of combined general anesthesia, resulting in reduced variability of severity of postresuscitation disease in laboratory animals; improving reproducibility of the model and its similarity with the clinical course of postresuscitation disease in humans in a clinical setting.

1 cl, 3 dwg, 1 ex