METHOD FOR SELECTING OPTIMAL DOSE AT WHICH PREPARATION EXHIBITS MAXIMUM ANTIOXIDANT ACTIVITY FOR INTRODUCTION INTO CONJUNCTIVAL CAVITY IN EYE DISEASES Russian patent published in 2024 - IPC G01N33/52 A01B5/00 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to ophthalmology and pharmacology, and can be used to select the optimal dose at which the preparation exhibits maximum antioxidant activity for introduction into the conjunctival cavity in eye diseases in an experiment. Lachrymal fluid is collected using filter paper, eluted with normal saline and centrifuged. Series of solutions of the analysed preparation is prepared by serial dilutions thereof with 0.05 M phosphate buffer solution pH 7.4 to concentrations of 0.015 to 14.54 mM. Solution of the analysed preparation is then added to the supernatant at ratio of 1:1 and the total antioxidant activity is assessed at the analysed concentrations of the preparation by a chemiluminescent method in a model system of haemoglobin – hydrogen peroxide – luminol. Optimal dose is selected at which the preparation exhibits maximum total antioxidant activity.

EFFECT: invention provides obtaining objective data on antioxidant potential of tear and test preparation with selection of optimal dose of preparation with antioxidant properties for application in the form of eye drops.

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