FIELD: biotechnology; medicine.
SUBSTANCE: invention relates to biotechnology and medicine, in particular to methods in vitro for determining the effectiveness of cell therapy containing retinal precursor cells (RPC), in the treatment of retinal degeneration. Method comprises incubating a first plurality of cells with a toxic compound and a conditioned medium, wherein the conditioned medium comprises a medium used in RPC cultivation; incubating at least a second plurality of cells with a toxic compound and a control medium; determining viability of a first and at least a second plurality of cells; and comparing the viability of the first and second plurality of cells, thereby determining the effectiveness of the cell therapy or treatment.
EFFECT: invention provides a reliable, cost-effective, time-saving in vitro assay for determining the effectiveness of cell therapy methods.
15 cl, 15 dwg, 9 ex
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