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2 831 404

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IPC

C12N15/21(2006-01-01)

C12N15/70(2006-01-01)

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Application

2023133370, 2023-12-12

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Start date

2023-12-12

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Actual filing date

2023-12-12

(45)

Published

2024-12-05

(72)

Inventor

Cherepanov Petr AlekseevichMikhajlova Tatyana GavrilovnaNikolaev Taras MarkovichKarpo Borislav StepanovichBobrysheva Irina VyacheslavovnaTsapenko Pavel VladimirovichTselikova Irina VladimirovnaShevchenko Dmitrij ValentinovichEfremova Asya Aleksandrovna

(73)

Holder

Obshchestvo S Ogranichennoj Otvetstvennostyu M" M")

RECOMBINANT PLASMID pFM-FLiC_syn, PROVIDING EXPRESSION OF RECOMBINANT FLAGELLIN PROTEIN SALMONELLA (FLiC), MONOPLASMID BACTERIAL STRAIN ESCHERICHIA COLI FM-FLiC_syn AND METHOD OF PRODUCING RECOMBINANT FLAGELLIN PROTEIN SALMONELLA (FLiC) Russian patent published in 2024 - IPC C12N15/21 C12N15/70

Abstract RU 2831404 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to recombinant plasmid pFM-FliC_syn, coding expression of Salmonella flagellin (FliC) protein, to a recombinant strain Escherichia coli FM-FliC_syn-producer of recombinant FliC and to a method of producing a recombinant flagellin protein and can be used as an adjuvant for vaccines. Recombinant strain of bacteria E. coli FM-FliC_syn is obtained by transformation of cells of the strain E. coli BL21 with recombinant plasmid DNA pFM-FliC_syn. Recombinant plasmid DNA pFM-FliC_syn, size 4244 bp, codes Salmonella flagellin (FliC) protein synthesis under control of lactose and tryptophan promoter and rrnBT1T2 transcription terminator and contains as a genetic marker a synthetic gene kan^R, which determines kanamycin resistance.

EFFECT: invention enables to obtain recombinant flagellin protein Salmonella (FliC) with high output and using a simplified technology.

3 cl, 8 dwg, 4 ex

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RU 2 831 404 C1

Authors

Cherepanov Petr Alekseevich

Mikhajlova Tatyana Gavrilovna

Nikolaev Taras Markovich

Karpo Borislav Stepanovich

Bobrysheva Irina Vyacheslavovna

Tsapenko Pavel Vladimirovich

Tselikova Irina Vladimirovna

Shevchenko Dmitrij Valentinovich

Efremova Asya Aleksandrovna

Dates

2024-12-05—Published

2023-12-12—Filed