FIELD: chemistry.
SUBSTANCE: invention relates to biotechnology and a recombinant strain of Escherichia coli bacteria - a producer of biologically active flagellin. The described strain is obtained by transformation of an E. coli BL21[DE3] cell culture with recombinant plasmid DNA pET151FliC, which is obtained based on a pET151FliC vector in which was embedded a fliC gene which codes biologically active flagellin, having a nucleotide sequence represented in Seq ID No 3. The strain is deposited in the Russian National Collection of Industrial Microorganisms (RCIM) of the Research Institute for Genetics and Selection of Industrial Microorganisms under No B-11369.
EFFECT: present solution has higher production capacity with respect to recombinant flagellin, which is an effective adjuvant.
1 dwg, 2 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| BACTERIAL STRAIN ESCHERICHIA COLI - PRODUCER OF RECOMBINANT EXTRACELLULAR DOMAIN OF PROTEIN GP | 2018 |
|
RU2701337C1 |
| DNA CODING FULL-SIZE HUMAN INTERLEUKIN-RECEPTOR ANTAGONIST 36, DNA CODING FULL-SIZE HUMAN INTERLEUKIN-RECEPTOR ANTAGONIST 36 WITH C-END POLIGISTIDINE TAG, PLASMID EXPRESSION VECTOR (VERSIONS), ESCHERICHIA COLI BL21 [DE3] STAR (PET-IL36RAF),-PRODUCER OF FULL-SIZE RECEPTOR ANTAGONIST 36 AND STRAIN OF BACTERIA ESCHERICHIA COLI BL21 [DE3] STAR (PET-IL36RAF-HIS),-PRODUCER OF FULL-SIZE RECEPTOR ANTAGONIST 36 WITH C-END POLIGISTIDINE TAG | 2014 |
|
RU2582569C2 |
| METHOD TO OBTAIN CHEMERIC RECOMBINANT PROTEIN fliC:pagN | 2015 |
|
RU2627602C2 |
| RECOMBINANT PLASMID DNA OF PTRX-TEVRS-PTH CODING A HYBRID PROTEIN CAPABLE OF PROTEOLYTIC SPLITTING TO FORM A FRAGMENT OF ENDOGENOUS HUMAN PARATHYROID HORMONE (1-34), AN ESCHERICHIA COLI BL21(DE3)/PTRX-TEVRS-PTH STRAIN - PRODUCER OF SAID PROTEIN AND A METHOD OF PRODUCING RECOMBINANT PTH (1-34) | 2019 |
|
RU2700452C1 |
| RECOMBINANT STRAIN Escherichia coli-PRODUCER OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS FROM Xanthomonas rubrilineans, METHOD FOR MICROBIOLOGICAL SYNTHESIS OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS BASED ON ABOVE SAID STRAIN | 2012 |
|
RU2499830C1 |
| STRAIN Escherichia coli - PRODUCER OF HYDROLASE OF ALPHA-AMINO ACIDS FROM Xanthomonas rubrilineans, AND METHOD FOR MICROBIOLOGICAL SYNTHESIS OF HYDROLASE OF ALPHA-AMINO ACIDS ESTERS BASED ON ABOVE SAID STRAIN | 2012 |
|
RU2502797C1 |
| HYBRID PROTEIN (VERSIONS), BACTERIAL STRAIN ESCHERICHIA COLI - HYBRID PROTEIN PRODUCER (VERSIONS) AND METHOD FOR PRODUCING METHIONINE-FREE HUMAN INTERFERON ALPHA-2 | 2011 |
|
RU2453604C1 |
| RECOMBINANT PLASMID DNA CODING FUSION PROTEIN L-HEP, ESCHERICHIA COLI STRAIN PRODUCER OF SAID PROTEIN AND METHOD OF PRODUCING RECOMBINANT PROTEIN | 2019 |
|
RU2716975C1 |
| PLASMID VECTOR pE-Trx-Aur, STRAIN ESCHERICHIA COLI FOR EXPRESSION OF ANTIMICROBIAL PEPTIDE AURELIN AND METHOD OF SAID PEPTIDE OBTAINING | 2009 |
|
RU2412999C1 |
| RECOMBINANT DNA, CODING HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR (G-CSF) AND RECOMBINANT PLASMID pAS017, PROVIDING G-CSF SYNTHESIS IN CELLS OF Escherichia coli | 2011 |
|
RU2529363C2 |
