METHOD FOR PREDICTION OF OUTCOME IN PATIENTS WITH GASTRIC CANCER AFTER COMBINED TREATMENT, INCLUDING NEOADJUVANT CHEMOTHERAPY ACCORDING TO FOLFOX6 OR FLOT REGIMENS AND SURGICAL TREATMENT Russian patent published in 2024 - IPC G01N33/50 G01N33/574 C12Q1/6804 C12Q1/6844 C12Q1/6876 C12Q1/6886 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to oncology, and can be used for prediction of outcome in patients with gastric cancer after combined treatment including neoadjuvant chemotherapy according to FOLFOX6 or FLOT regimens and surgical treatment. Tumour tissue is used to obtain cDNA on a total RNA matrix using a reverse transcription reaction. PD-L1 and PDK1 genes are amplified. PD-L1 and PDK1 gene expression is assessed by method 2^-ΔΔCt relative to GAPDH gene. Obtained values are substituted into the regression function formula and the probability of a favourable outcome P is calculated. If P<0.061, a favourable outcome is predicted. If P>0.061, an unfavourable outcome is predicted.

EFFECT: method provides more accurate and informative prediction of the outcome in the patients with gastric cancer following the combined treatment including neoadjuvant chemotherapy according to the FOLFOX6 or FLOT regimens and surgical treatment by assessing the expression of PD-L1 and PDK1 genes in the tumour tissue.

1 cl, 6 dwg, 2 ex