METHOD OF PRODUCING CELL PREPARATION BASED ON ACTIVATED LYMPHOCYTES FROM LEUKOCYTE FILTERS Russian patent published in 2025 - IPC A61K38/20 C12N5/781 C12N5/783 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, namely to a method for producing a cell preparation based on activated lymphocytes from leukocyte filters. Method comprises recovery of lymphocytes on ficoll gradient, activation in culture flasks or plates in nutrient medium, resuspension of activated cytotoxic lymphocytes in supernatant and intracutaneous paravertebral introduction from 3rd day of activation, updating half the volume of the nutrient medium every 48 hours and distributing the supernatants into ampoules with volume of 1.3-2 ml, storage in a freezer for subsequent use in outpatient treatment.

EFFECT: method enables to obtain large doses – more than 300 million cells at a time – from one filter after activation of activated HLA-DR+ lymphocytes: T-cytotoxic cells CD3+CD8+ and NKT- cells CD3+CD16+CD56+ with high viability >96%, as well as to obtain supernatants containing high doses of cytokines produced by lymphocytes during activation in vitro: IL-2 >250 IU/ml, IL-6 >30 IU/ml, IL-10 >180 IU/ml, IFN-γ >550 IU/ml, TNF-α >100 IU/ml.

2 cl, 6 dwg, 1 ex