FIELD: biotechnology.
SUBSTANCE: disclosed is an integrative expression cassette for the expression of the RmAmyA gene encoding a recombinant α-amylase from R. miehei, comprising a PAOM promoter, a nucleotide sequence of a signal peptide, RmAmyA gene coding recombinant α-amylase from R. miehei, transcription terminator, selective marker for selection of transformants in yeast cells, region of integration, which is a fragment of nucleotide sequence M1 from chromosome K. mondaviorum VKPM Y-4330. Also disclosed is use of said integrative expression cassette for producing a transformant of yeast species K. mondaviorum, producing recombinant α-amylase from R. miehei and containing the RmAmyA gene.
EFFECT: invention enables to obtain recombinant protein producer strains based on methylotrophic yeast Komagataella mondaviorum.
2 cl, 2 dwg, 2 tbl, 5 ex
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