FIELD: biotechnology.
SUBSTANCE: invention relates to microbiology and biotechnology. Disclosed is a recombinant yeast strain Pichia pastoris VKPM Y-4484, which produces phytase. Above strain contains an optimized sequence of a gene encoding phytase of Citrobacter freundii as a part of a chromosome.
EFFECT: strain synthesizes Citrobacter freundii phytase in concentration of 1403 units/ml of culture fluid.
1 cl, 1 dwg, 1 ex
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Authors
Dates
2019-09-26—Published
2018-12-24—Filed