FIELD: microbiology and biotechnology.
SUBSTANCE: invention relates to microbiology and biotechnology. Yeast strain Komagataella phaffii YLM9 with an inactivated LEU2 gene is proposed as a recipient for constructing strains producing heterologous proteins. The specified strain is deposited under the number RNCIM Y-5014.
EFFECT: invention provides obtaining a recipient strain, which makes it possible to obtain highly productive marker-free strains producing heterologous proteins.
1 cl, 4 dwg, 2 tbl, 3 ex
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