FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology, in particular to selective production of (-)-ambroxide. Disclosed is a method of producing (-)-ambroxide or a mixture containing (-)-ambroxide, where the mixture of isomers containing (3E,7E)-homoparnesol (EEN) is enzymatically converted into (-)-ambroxide or into a mixture containing (-)-ambroxide, using the enzyme squalene-hopene-cyclase/homopharanesol-ambroxide-cyclase (SHC/HAC), containing an amino acid sequence having at least 30% identity with SEQ ID NO: 1, in suitable reaction conditions. Mixture of isomers containing EEH is selected from at least one of [EE:EZ], [EE:ZE] and [EE:EZ:ZE]. (-)-Ambroxide is present in solid phase in form of crystals or in amorphous form.
EFFECT: method leads to obtaining product containing 80−97% (-)-ambroxide in solid form, which is used in a flavoring product, or cosmetic product, or consumer product.
24 cl, 25 dwg, 26 tbl, 23 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PRODUCING (-)-AMBROXIDE, A REACTION PRODUCT CONTAINING (-)-AMBROXIDE, AND USE THEREOF | 2016 |
|
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| IMPROVED SINGLE VARIABLE IMMUNOGLOBULIN DOMAINS BINDING TO SERUM ALBUMIN | 2017 |
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| FUSION POLYPEPTIDE CONTAINING GDF15 AND POLYPEPTIDE REGION CAPABLE OF O-GLYCOSYLATION | 2020 |
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| HETERODIMERIC FC-FUSED PROTEINS IL15/IL15Rα | 2017 |
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