FIELD: biotechnology.
SUBSTANCE: invention relates to production of a compound with a sweet taste. Disclosed is a method of producing compound 1, comprising contacting mogroside IIIE with an enzyme capable of catalyzing the production of compound 1 from mogroside IIIE, in the presence of glucose. Enzyme is one or more of cyclomaltodextrin-glucanotransferases (CGTases), dextransucrases and dextranases. Compound 1 exhibits tolerance to hydrolysis by certain hydrolyzing enzymes, even if such enzymes demonstrate the possibility of hydrolysis of hyperglycosylated mogrosides to mogroside IIIE.
EFFECT: invention provides improved purity of compound 1 due to reduction of undesirable levels of mogroside VI, mogroside V and mogroside IV.
19 cl, 43 dwg, 5 tbl, 68 ex
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