FIELD: biotechnology.
SUBSTANCE: what is created is a plasmid DNA cartridge pVAX-C3-PolyA used for insertion of a target gene for forming an mRNA synthesis matrix having the nucleotide sequence SEQ ID NO: 1 with size 3171 bp, molecular weight 1.9 MDa. Created is Plasmid DNA matrix pVAX-C3-PvBD-PolyA for mRNA synthesis, obtained using a plasmid DNA cartridge pVAX-C3-PolyA according to cl. 1, having the nucleotide sequence SEQ ID NO: 2 with size 3903 bp, molecular weight 2.3 MDa, including target gene coding chimeric protein 176-RBD having amino acid sequence SEQ ID NO: 4 and coordinates 701 to 1432. What is prepared is a polynucleotide vaccine representing mRNA-C3-RBD-PolyA molecules inducing SARS-CoV-2-specific antibodies having the nucleotide sequence SEQ ID NO: 3, synthesized by plasmid DNA matrix pVAX-RBD-C3-RBD-PolyA according to cl. 2, obtained using a plasmid DNA cartridge pVAX-C3-PolyA according to cl. 1.
EFFECT: development of a platform in the form of a DNA cartridge and a based DNA matrix for mRNA synthesis.
3 cl, 9 dwg, 5 ex
