MARKED J THYROXINE DERIVATIVES AS REAGENT FOR RADIOIMMUNE ANALYSIS AND THYROXINE/AS DERIVATIVES INTERMEDIATES FOR PRODUCING MARKED J THYROXINE DERIVATIVES Russian patent published in 1994 - IPC

FIELD: radioactively marked compounds; more particularly ¹²⁵J thyroxine derivatives of the general formula 1: a) - N; R₂- -CH₃; b) R₁- -OH ; c) which can be used as reagents for radioimmune analysis. SUBSTANCE: new semiproducts of the general formula II: are wherein a) ; R₄- -CH₃; b) R₃- -OH; R₄-(CH₂)₂-C₆H₄-OH; c) ; R₄- -(CH₂)₂-C₆H₄-OH, prepared by reaction of N-acetylthyroxine N-oxysuccinimide ester and histamine dihydrochloride in aqueous in the presence of triethylamine. The compounds of the formula I are synthesized from said semiproducts by treatment with Na ¹²⁵J in the presence of phosphate buffer and chloroamine T in water followed by elution with 50% methanol. Radiochemical yield, % ; molar activity, KC/mole; radiochemical purity (% ): (a) 30; 1500-2000; > 95; (b) 35; 1500-2000: > 95; (c) 20; 3700-4000; > 90. New compounds are capable of being with antibodies to thyroxine and are not capable of being bonded to endogenous thyroxine binding protein. EFFECT: new semiproducts for the synthesis of marked ¹²⁵J semiproducts of the formula 1. 3 cl, 1 dwg, 2 tbl