FIELD: medicine.
SUBSTANCE: recombinant alpha 16-interferon is made by cultivation of recombinant strain of yeast Pichia pastoris All Russian Collection of Industrial Microorganisms Y-2949 in a nutrient medium. It is followed with induced synthesis of target protein and cell removal. Then the culture medium is concentrated with settling of target protein at 60% ammonium sulphate saturation. Then said target protein is consistently purified on sephacryl HR 100 and on diethylaminoethyl-cellulose with 0.5% detergent Tween 20 added.
EFFECT: invention allows simplifying technology of recombinant human alpha 16-interferon and improving effectiveness of purification of target protein.
5 cl, 2 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PRODUCING RECOMBINANT PROTEIN, MODIFIED THROUGH ATTACHMENT OF HUMAN ALBUMIN FROM YEAST CULTURE FLUID | 2007 |
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RU2373286C2 |
| SOLUTION FOR INJECTION, RECOMBINANT PLASMID DNA pSX50 ENCODING SYNTHESIS OF HUMAN RECOMBINANT ALPHA-2b INTERFERON, STRAIN Escherichia coli SX50 AS INDUSTRIAL STRAIN-PRODUCER OF HUMAN RECOMBINANT ALPHA-2b INTERFERON AND METHOD FOR INDUSTRIAL PREPARING INTERFERON ALPHA-2b | 2006 |
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RU2319502C1 |
| STRAIN OF YEAST PICHIA PASTORIS AS PRODUCER OF LEUKOCYTE HUMAN INTERFERON-16, RECOMBINANT PLASMID DNA PHIN AND METHOD FOR ITS CONSTRUCTION | 2002 |
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RU2203950C1 |
| HUMAN INTERFERON-ALPHA-TWO AQUEOUS SOLUTION FOR INJECTION | 2002 |
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RU2238758C2 |
| YEAST STRAIN Pichia pastoris PS106(pHIG) AS PRODUCER OF HUMAN IMMUNE INTERFERON, RECOMBINANT PLASMID pHIG AND METHOD FOR ITS DESIGN | 2006 |
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RU2315806C1 |
| COMPOSITION OF AQUEOUS SOLUTION OF RECOMBINANT HUMAN INTERFERON ALPHA-2 FOR RECTAL ADMINISTRATION | 2014 |
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RU2564951C1 |
| METHOD FOR PREPARING RECOMBINANT INTERFERON ALPHA-2b AND INTERFERON-CONTAINING PREPARATION (VARIANTS) | 2005 |
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RU2294372C1 |
| RECOMBINANT PLASMID DNA ENCODING CHIMERIC INTERFERON ALPHA2b, RECOMBINANT STRAIN OF YEAST P. PASTORIS X33 - PRODUCER OF CHIMERIC INTERFERON ALPHA2b, AND METHOD FOR PRODUCING THIS PROTEIN | 2020 |
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RU2764787C1 |
| RECOMBINANT PLASMID, METHOD FOR ITS CONSTRUCTION AND YEAST STRAIN KOMAGATAELLA PASTORIS - PRODUCER OF IMMUNE INTERFERON GAMMA OF A DOG | 2020 |
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RU2756852C2 |
| METHOD OF PREPARING HUMAN ALPHA-INTERFERON | 1989 |
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SU1640996A1 |
