Show metadata Hide metadata

(19)

(11)

2 373 286

(13)

(51)

IPC

C12P21/02(2006-01-01)

C12N15/62(2006-01-01)

C12N1/19(2006-01-01)

C12N15/00(2006-01-01)

(21) (22)

Application

2007143930/13, 2007-11-29

(24)

Start date

2007-11-29

(22)

Actual filing date

2007-11-29

(45)

Published

2009-11-20

(72)

Inventor

Padkina Marina VladimirovnaKarabel'Skij Aleksandr VladimirovichZinov'Eva Julija Grigor'EvnaSambuk Elena ViktorovnaSmirnov Mikhail Nikolaevich

(73)

Holder

Federal'Noe Gosudarstvennoe Obrazovatel'Noe Uchrezhdenie Vysshego Professional'Nogo Obrazovanija Sankt-Peterburgskij Gosudarstvennyj UniversitetObshchestvo S Ogranichennoj Otvetstvennost'Ju

METHOD OF PRODUCING RECOMBINANT PROTEIN, MODIFIED THROUGH ATTACHMENT OF HUMAN ALBUMIN FROM YEAST CULTURE FLUID Russian patent published in 2009 - IPC C12P21/02 C12N15/62 C12N1/19 C12N15/00

Abstract RU 2373286 C2

FIELD: chemistry; biochemistry.

SUBSTANCE: invention relates to biotechnology, specifically to a method of producing recombinant protein human albumin-interleukin-2 or recombinant protein human albumin-alpha 16-interferon, modified by attachment of human albumin. The method involves technology of culturing yeast strain Pichia pastoris PS106/pPIC9HAbIL-2 or yeast strain Pichia pastoris PS106/pPIC9HAbIFNa-16 in modified culture medium BMGY, after which induction synthesis of target proteins is carried out at low temperature. Further, cells are removed and the medium is concentrated. Target proteins are then precipitated using ammonium sulphate or polyethyleneglycol 3350. Target proteins are then separated by gel filtration on Sephacryl HR 200 or BioRad P-300 sorbents. Finally, affinity chromatography is then done on Cibacron F3GA sorbent.

EFFECT: invention simplifies and increases efficiency of the technology of purifying target proteins, and also allows for obtaining biologically active hybrid proteins, suitable for making medicinal agents.

3 cl, 1 tbl, 5 ex

Similar patents RU2373286C2

Title	Year	Author	Number
METHOD FOR MAKING RECOMBINANT HUMAN ALPHA 16-INTERFERON AND PHARMACEUTICAL COMPOSITION FOR TREATING VIRAL DISEASES BASED ON RECOMBINANT HUMAN ALPHA 16-INTERFERON	2007	Padkina Marina Vladimirovna Dobrogorskaja Marina Vladimirovna Karabel'Skij Aleksandr Vladimirovich Sambuk Elena Viktorovna Smirnov Mikhail Nikolaevich	RU2380405C2
HYBRID PROTEIN HAVING PROLONGED ACTION, BASED ON RECOMBINANT HUMAN INTERFERON ALPHA-2 (VARIANTS), METHOD OF ITS PRODUCTION AND STRAIN OF Saccharomyces cerevisiae FOR IMPLEMENTING THIS METHOD (VERSIONS)	2013	Kozlov Dmitrij Georgievich Cheperegin Sergej Ehduardovich Gubajdullin Irek Il'Jasovich Efremov Boris Dmitrievich Kotlova Elena Konstantinovna Rjabichenko Viktor Vasil'Evich Chestukhina Galina Georgievna Jarotskij Sergej Viktorovich	RU2515913C1
YEAST Pichia pastoris X-33/2albumin STRAIN FOR SECRETION OF HUMAN ALBUMIN	2005	Novikov Dmitrij Viktorovich Pervushkin Petr Mikhajlovich Novikov Viktor Vladimirovich	RU2306333C2
HYBRID PROTEIN (VERSIONS), BACTERIAL STRAIN ESCHERICHIA COLI - HYBRID PROTEIN PRODUCER (VERSIONS) AND METHOD FOR PRODUCING METHIONINE-FREE HUMAN INTERFERON ALPHA-2	2011	Kozlov Dmitrij Georgievich Jakovenko Andrej Romanovich Tezov Vladimir Adol'Fovich	RU2453604C1
RECOMBINANT PLASMID DNA ENCODING CHIMERIC INTERFERON ALPHA2b, RECOMBINANT STRAIN OF YEAST P. PASTORIS X33 - PRODUCER OF CHIMERIC INTERFERON ALPHA2b, AND METHOD FOR PRODUCING THIS PROTEIN	2020	Beklemishev Anatolij Borisovich Pykhtina Mariya Borisovna	RU2764787C1
PLASMID VECTOR PRH15A FOR PRODUCING METHIONINE-FREE INTERFERON ALPHA-2B, BACTERIAL STRAIN ESCHERICHIA COLI BL21 DE3 - PRODUCER OF METHIONINE-FREE INTERFERON ALPHA-2B AND METHOD FOR PRODUCING METHIONINE-FREE INTERFERON ALPHA-2B	2017	Kudling Tatyana Viktorovna Karasev Maksim Mikhajlovich Kolobov Aleksej Aleksandrovich Lisitskaya Veronika Igorevna Romanov Nikolaj Ivanovich Savin Ilya Igorevich Elgina Alena Fedorovna Batarin Vladimir Ilich Gorbunova Irina Nikolaevna Antipova Tatyana Olegovna Denisenko Elizaveta Sergeevna Martyushin Sergej Vasilevich Ishchenko Aleksandr Mitrofanovich	RU2697375C2
FUSION PROTEIN, ESCHERICHIA COLI STRAIN BEING FUSION PROTEIN PRODUCER AND METHOD FOR PRODUCING METHIONINE-FREE HUMAN INTERFERON ALPHA-2b OF SUCH FUSION PROTEIN	2010	Kozlov Dmitrij Georgievich Cheperegin Sergej Ehduardovich Gubajdullin Irek Il'Jasovich Jakovenko Andrej Romanovich Kazarov Aleksandr Aleksandrovich	RU2441072C1
STRAIN OF BACTERIA Escherichia coli JM109/pHINS21 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN AND METHOD FOR PRODUCTION OF HUMAN PROINSULIN	2007	Rodionov Petr Ivanovich Rodionov Petr Petrovich Shmatchenko Vadim Vasil'Evich Stepanov Aleksej Vjacheslavovich Bajdus' Aleksandr Nikolaevich Shmatchenko Natal'Ja Anatol'Evna Gorkun Taras Alekseevich	RU2376368C2
STRAIN OF YEAST PICHIA PASTORIS AS PRODUCER OF LEUKOCYTE HUMAN INTERFERON-16, RECOMBINANT PLASMID DNA PHIN AND METHOD FOR ITS CONSTRUCTION	2002	Padkina M.V. Parfenova L.V. Sambuk E.V. Smirnov M.N.	RU2203950C1
RECOMBINANT PLASMID, METHOD FOR ITS CONSTRUCTION AND YEAST STRAIN KOMAGATAELLA PASTORIS - PRODUCER OF IMMUNE INTERFERON GAMMA OF A DOG	2020	Muzaev Dmitrij Mikhajlovich Padkina Marina Vladimirovna	RU2756852C2

RU 2 373 286 C2

Authors

Padkina Marina Vladimirovna

Karabel'Skij Aleksandr Vladimirovich

Zinov'Eva Julija Grigor'Evna

Sambuk Elena Viktorovna

Smirnov Mikhail Nikolaevich

Dates

2009-11-20—Published

2007-11-29—Filed