FIELD: chemistry.
SUBSTANCE: method of obtaining recombinant human lactoferrin, free of lipopolysaccarides, includes ion-exchange chromatography of transgenic goats on strong cation-exchanger, carrying sulphopropyl groups, elution of lactoferrin in sodium chloride gradient, desalination, with the following application of step-by-step washing of sorbent with sodium acetate buffer, which contains sodium chloride and ethanol in order to remove lipopolisaccharides, bound with lactoferrin, freeze-drying under specified conditions.
EFFECT: method makes it possible to obtain recombinant human lactoferrin, free of lipopolysaccharides.
1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PRODUCING LACTOFERRIN | 2018 |
|
RU2717340C1 |
| METHOD FOR LACTOFERRIN ISOLATION AND PURIFICATION FROM DAIRY RAW MATERIALS | 2016 |
|
RU2634859C1 |
| APPLICATION OF A FLOW-THROUGH FILTERING CENTRIFUGE FOR THE EXTRACTION OF LACTOFERRIN FROM DAIRY RAW MATERIALS | 2021 |
|
RU2780347C1 |
| METHOD OF SEPARATING LACTOFERRIN OF HUMAN AND GOAT BY MEANS OF DIFFERENTIAL IMMUNOAFFINITY CHROMATOGRAPHY USING SINGLE-DOMAIN MINI-ANTIBODIES | 2014 |
|
RU2553515C1 |
| METHOD OF LACTOFERRIN OBTAINING FROM DAIRY RAW MATERIALS | 2008 |
|
RU2390253C1 |
| METHOD FOR PRODUCING LACTOFERRIN, LACTOFERRIN-CONTAINING FRACTION AND USING IT | 2010 |
|
RU2579661C2 |
| METHOD OF HUMAN RECOMBINANT TUMOR NECROSIS ALPHA-FACTOR PREPARING | 1997 |
|
RU2144958C1 |
| METHOD OF ISOLATION AND PURIFICATION OF INSULIN OR ITS BIOTECHNOLOGICAL PRECURSORS | 1994 |
|
RU2081122C1 |
| METHOD OF PREPARING HUMAN RECOMBINANT GRANULOCYTIC COLONY-STIMULATING FACTOR | 2001 |
|
RU2201962C2 |
| METHOD FOR LARGE-SCALE PRODUCTION, SEPARATION AND PURIFICATION OF RECOMBINANT HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR | 2010 |
|
RU2487885C2 |
