FIELD: medicinal biotechnology. SUBSTANCE: method involves determination of antigens using conjugate of bacteriophage 0X 174 and Fab′-fragments of specific monoclonal antibodies. Antibodies are treated with iodoacetamide excess. Examined material is transferred in indicator culture of microorganism, lysis zones are detected automatically, and results of reaction are recorded. Sensitivity of determination is 1,5-3 IU/ml for γ-interferon and 1 IU/ml for a2-interferon. EFFECT: enhanced sensitivity of assay.
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR EVALUATING PHAGOIMMUNOASSAY RESULTS | 2006 |
|
RU2347225C2 |
| METHOD OF PREPARING COMPLEX OF LEUKOCYTE PROTEINASE INHIBITORS | 1995 |
|
RU2086650C1 |
| METHOD FOR PREVENTING BRUCELLOSIS | 1992 |
|
RU2037319C1 |
| METHOD FOR TREATMENT OF LEGIONELLOUS PNEUMONIA | 1986 |
|
RU2008019C1 |
| METHOD OF SERUM DIAGNOSIS OF INFECTION HELICOBACTER PYLORI BY IMMUNOENZYME ANALYSIS | 2001 |
|
RU2196993C1 |
| RECOMBINANT PLASMID plFN-αF-Pa CODING SYNTHESIS OF LEUCOCYTAL HUMAN INTERFERON AND STRAIN E COLI - PRODUCER OF LEUCOCYTAL HUMAN INTERFERON αF | 1984 |
|
SU1312962A1 |
| DIAGNOSTIC TECHNIQUE FOR PSEUDOTUBERCULOSIS | 2009 |
|
RU2429480C1 |
| METHOD OF ANTIBRUCELLOSIS SERUM PREPARING | 1993 |
|
RU2101030C1 |
| STRAIN OF HUMAN CULTURED CELLS HTHIY27 - A PRODUCER OF HUMAN IMMUNODEFICIENCY VIRUS | 1994 |
|
RU2067116C1 |
| METHOD OF PREPARING SOLUBLE CONJUGATES OF BIOLOGICALLY ACTIVE SUBSTANCES | 2000 |
|
RU2196605C2 |
