FIELD: biotechnology, genetic engineering. SUBSTANCE: strain of bacterium Bacillus sphaericus No. 209 is isolated from the soil by screening in the search of strains - producers of site-specific endonucleases among Bacillus species. Enzyme Bsh 451 recognizes and splits nucleotide sequence 5′-G(A/T)GC-(A/T)-C-3′. Strain grows on the simple low-cost monocomponent medium, which is prepared on the basis of fish-hydrolyzate at 30 C under effective aeration up to achievement of stationary growth phase. The yield of the end enzyme is 30000 U/g wet biomass. Specific activity of enzyme Bsh 451 is 10000 U/ml. EFFECT: enhanced yield of enzyme activity.
| Title | Year | Author | Number |
|---|---|---|---|
| STRAIN OF BACTERIA BACILLUS BREVIS - A PRODUCER OF RESTRICTASE BBVI 21 | 0 |
|
SU1694647A1 |
| STRAIN OF BACTERIUM BACILLUS STEAROTHERMOPHILUS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5′-CC(A/T)-GG-3′ | 1992 |
|
RU2038380C1 |
| STRAIN OF BACTERIUM BACILLUS PUMILIS PRODUCING RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5′- CCTNAGC- 3′ | 1992 |
|
RU2021373C1 |
| 0 |
|
SU1784642A1 | |
| STRAIN OF BACTERIUM PHOTOBACTERIUM PHOSPHOREUM - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING NUCLEOTIDE SEQUENCE 5'-GG(T/C)(AG)CC-3' | 1994 |
|
RU2110573C1 |
| STRAIN OF BACILLUS SPECIES 21 BACTERIA AS PRODUCER OF SITE-SPECIFIC ENDONUCLEASE BASE 21 | 0 |
|
SU1518372A1 |
| STRAIN OF BACTERIUM BACILLUS PUMILUS - A PRODUCER OF RESTRICTION ENDONUCLEASE BPU 19-1 | 1993 |
|
RU2046142C1 |
| METHOD FOR OBTAINING BIOMASS OF STREPTOMYCES ENRICHED IN RESTRICTION ENDONUCLEASE SFI I | 0 |
|
SU1701745A1 |
| STRAIN OF BACTERIA Kocuria rasea-PRODUCER OF SITE-SPECIFIC ENDONUCLEASES KroI | 2009 |
|
RU2394099C1 |
| STRAIN OF BACTERIUM BACILLUS STEAROTHERMOPHILUS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING NUCLEOTIDE SEQUENCE 5'-GGTNACC-3' | 1996 |
|
RU2115728C1 |
