STRAIN OF BACTERIUM BACILLUS PUMILUS - A PRODUCER OF RESTRICTION ENDONUCLEASE BPU 19-1 Russian patent published in 1995 - IPC

Abstract RU 2046142 C1

FIELD: microbiology, biotechnology. SUBSTANCE: product: restriction endonuclease recognizing and splitting the nucleotide sequence 5′-CCATGG-3′. New strain of Bacillus pumilus (ВКПМ B-6308) is proposed. Strain is a producer restrictase Bpu 19-1 isolated from the soil after screening of restrictase producers. The yield of enzyme is 5000 U/g wet biomass, specific activity of the end enzyme is 20000 U/ml. Restrictase Bpu 19-1 is an isoschizomer of restrictase NspSA III and can replace the latter in all genetic engineering investigations. EFFECT: high yield and activity of enzyme. 1 dwg

Similar patents RU2046142C1

Title	Year	Author	Number
STRAIN OF BACTERIUM BACILLUS BADIUS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING THE NUCLEOTIDE SEQUENCE 5′-(A/T)CCGG(A/T)-3′	1993	Degtjarev S.Kh. Repin V.E. Rechkunova N.I. Shevchenko A.V. Abdurashitov M.A.	RU2053299C1
STRAIN OF BACTERIUM BACILLUS COAGULANS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING THE NUCLEOTIDE SEQUENCE 5′-GATNNNN-ATC-3′	1994	Repin V.E. Tereshchenko T.A. Lebedev L.R.	RU2057806C1
STRAIN OF BACTERIUM BACILLUS SCHLEGELII - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING THE NUCLEOTIDE SEQUENCE 5'-CCNNNNNNNGG-3'	1994	Repin V.E. Tereshchenko T.A. Lebedev L.R.	RU2073717C1
STRAIN OF BACTERIUM BACILLUS PUMILIS PRODUCING RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5′- CCTNAGC- 3′	1992	Degtjarev S.Kh. Rechkunova N.I. Repin V.E.	RU2021373C1
STRAIN OF BACTERIUM ACINETOBACTER CALCOACETICUS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING THE NUCLEOTIDE SEQUENCE 5'-GGTACC-3'	1993	Repin V.E. Degtjarev S.Kh. Rechkunova N.I.	RU2034922C1
STRAIN OF BACTERIUM BACILLUS COAGULANS - A PRODUCER OF SITE-SPECIFIC ENDONUCLEASE WHICH RECOGNIZES AND SPLITS THE NUCLEOTIDE SEQUENCE 5′-CTCTTC-3′	1993	Repin V.E. Tereshchenko T.A. Lebedev L.R.	RU2054040C1
STRAIN OF BACTERIUM BACILLUS SPECIES - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5'-(A/G)-CCGG-(T/C)-3'	1992	Repin Vladimir Evgen'Evich	RU2037522C1
STRAIN OF BACTERIA BACILLUS COAGULANTS BEING PRODUCER OF SIDE SPECIFIC ENDONUCLEASE WHICH IS ENABLE TO IDENTIFY AND TO CLEAVE SERIES OF 5′-CT(A/G)(T/C)AG-3′ NUCLEOTIDES	1993	Repin V.E.	RU2040540C1
STRAIN OF BACTERIUM BACILLUS STEAROTHERMOPHILUS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5′-CC(A/T)-GG-3′	1992	Repin Vladimir Evgen'Evich	RU2038380C1
STRAIN OF BACTERIUM PHOTOBACTERIUM PHOSPHOREUM - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING NUCLEOTIDE SEQUENCE 5'-GG(T/C)(AG)CC-3'	1994	Repin V.E. Puchkova L.I. Rodicheva Eh.K. Vydrjakova G.A.	RU2110573C1

RU 2 046 142 C1

Authors

Repin V.E.

Degtjarev S.Kh.

Rechkunova N.I.

Dates

1995-10-20—Published

1993-08-16—Filed