STRAIN OF BACTERIUM BACILLUS STEAROTHERMOPHILUS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING NUCLEOTIDE SEQUENCE 5'-GGTNACC-3' Russian patent published in 1998 - IPC

Abstract RU 2115728 C1

FIELD: biochemistry, enzymology. SUBSTANCE: strain Bacillus stearothermophilus - a producer of restrictase Bst T101 is isolated from the soil by screening of restrictase producers. Enzyme recognizes and cleaves nucleotide sequence 5'-GGTNACC-3'. Enzyme yield is 4 000 U/g wet biomass, specific activity of the end enzyme is 10 000 U/ml. Restrictase BSt T101 is an isoschizomer of restrictase 5′-GGTNACC-3′ and can replace the latter in all genetic engineering works. Restrictase Bst T101 can be used for investigation of protein-nucleic acid interaction specificity. EFFECT: strain and enzyme indicated above, high yield and specific activity of an enzyme. 1 dwg

Similar patents RU2115728C1

Title	Year	Author	Number
STRAIN OF BACTERIUM DELEYA AQUAMARINA - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5'-GTGCAC-3'	1996	Repin V.E. Puchkova L.I. Ushakova T.A. Mikhajlova V.K. Repina M.V. Litosh V.G.	RU2105811C1
STRAIN OF BACTERIUM BACILLUS STEAROTHERMOPHILUS 22 AS PRODUCER OF SITE-SPECIFIC RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5'-CCNNNNN^NNGG-3' AND ELICITING GROWTH-STIMULATING ACTIVITY WITH RESPECT TO CULTURAL PLANT SEEDS	2002	Puchkova L.I. Andreeva I.S. Ushakova T.A. Radionenko Ju.V. Repin V.E. Polushkina A.F.	RU2238972C2
STRAIN OF BACTERIUM STREPTOMYCES SPECIES SJ-12 - PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING NUCLEOTIDE SEQUENCE 5'-CTGCAG-3'	1997	Puchkova L.I. Andreeva I.S. Ushakova T.A. Krivopalova G.N. Repin V.E.	RU2135581C1
BACTERIUM STRAIN SPHINGOBACTERIUM MIZUTAE-32 AS PRODUCER OF SPML RESTRICTION ENDONUCLEASE, RECOGNIZING AND CLEAVING 5'-AT'CGAT-3' NUCLEOTIDE SEQUENCE	2003	Puchkova L.I. Radionenko Ju.V. Ushakova T.A. Repin V.E.	RU2266323C2
METHOD OF ENDONUCLEASE RESTRICTION BSE 21 I PREPARING	1999	Mikhajlova V.K. Puchkova L.I. Kuvshinov V.N. Ushakova T.A. Repin V.E.	RU2184777C2
STRAIN OF BACTERIUM BACILLUS STEAROTHERMOPHILUS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5′-CC(A/T)-GG-3′	1992	Repin Vladimir Evgen'Evich	RU2038380C1
STRAIN OF BACTERIUM Paenibacillus sp, PRODUCER OF RESTRICTION ENDONUCLEASE Psp 1009I	2007	Puchkova Larisa Ivanovna Andreeva Irina Sergeevna Saranina Irina Vasil'Evna Pechurkina Natal'Ja Ivanovna Afonina Veronika Sergeevna Repin Vladimir Evgen'Evich	RU2340663C1
STRAIN OF BACTERIUM BACILLUS COAGULANS - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING THE NUCLEOTIDE SEQUENCE 5′-GATNNNN-ATC-3′	1994	Repin V.E. Tereshchenko T.A. Lebedev L.R.	RU2057806C1
STRAIN OF BACTERIUM BACILLUS SPECIES - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5'-(A/G)-CCGG-(T/C)-3'	1992	Repin Vladimir Evgen'Evich	RU2037522C1
STRAIN OF BACTERIUM BACILLUS SCHLEGELII - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING THE NUCLEOTIDE SEQUENCE 5'-CCNNNNNNNGG-3'	1994	Repin V.E. Tereshchenko T.A. Lebedev L.R.	RU2073717C1

RU 2 115 728 C1

Authors

Andreeva I.S.

Puchkova L.I.

Saranina I.V.

Lokhova I.A.

Repin V.E.

Dates

1998-07-20—Published

1996-06-14—Filed