STRAIN OF BACTERIUM BACILLUS PUMILIS PRODUCING RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5′- CCTNAGC- 3′ Russian patent published in 1994 - IPC

Abstract RU 2021373 C1

FIELD: biotechnology. SUBSTANCE: strain Bacillus pumilis VKPM B-5464 is detected which is a producer of restrictase Bpu101. This enzyme recognizes and cleaves nucleotide sequence 5′- CCTNAGC- 3′. Starin is isolated from the soil after restrictase producers screening. Strain grows on the simple, available and cheap medium. Yield of enzyme is 200 U/g wet biomass, specific activity of enzyme is 100 U/ml. Strain provides restrictase production showing unique site of recognition and cleaving of nucleotide sequence 5′- CCTNAGC- 3′. EFFECT: detection of bacterial strain indicated above. 1 dwg, 1 tbl

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RU 2 021 373 C1

Authors

Degtjarev S.Kh.

Rechkunova N.I.

Repin V.E.

Dates

1994-10-15—Published

1992-06-29—Filed