METHOD FOR PRODUCING PROBIOTICS FOR ANIMALS AND POULTRY Russian patent published in 1994 - IPC

FIELD: biotechnology. SUBSTANCE: method involves modifications in composition of microflora, ratio of cultures and in accumulation their biomass. Concentrated protein hydrolyzate of skimmed milk is used as fodder substrate and as drying medium simultaneously. Said hydrolyzate is prepared by hydrolysis of protosubtiline having activity 70-80 units at dose 0.020-0.030 mass % , the process is carried out within 6-8 h. Bifidobacteria and mesophile lactic acid streptococcus (Lactococcus lactis sulsp. diacetillactis) are accumulated together, the process is carried out on nutrient medium at 39-41 C, initial dose of components is 8-10 mass % and 0.01-0.1 mass % respectively. Nutrient medium contains, mass %: glucose 0.4-0.5, sodium acetate 0.2-0.3, potassium hydrophosphate or potassium dihydrophosphate 0.1-0.2, corn extract 1.8-2.0, sodium citrate 0.4-0.5, ferric sulfate 0/03-0.05. Inoculate of bifidobacteria and lactic acid streptococcus or cell biomass together with separately prepared ferment of Bacillus acidophilus are added into protein hydrolyzate at ratio 5-20 mass % and 1-5 mass %, said process is followed by spray drying. Mentioned above hydrolyzate is condensed till dry substance content in it is 38-42%. EFFECT: increases biological value of the product, decreases its cost.