FIELD: medicine. SUBSTANCE: euglobulin fraction is isolated of patient's blood with the help of dialysis. Dialyzate is centrifuged, precipitate being washed 3 times by 0.01 M phosphate buffer. Then 0.14 M solution of sodium chloride is added and additional centrifuging is carried out. Thus prepared precipitate is dissolved in 0.15 M solution of phosphate buffer. The process is followed by column gel chromatography. If on lines of eluted components the second peak of protein appears then atherosclerosis is diagnosed. Proposed method is used in pre-clinical stage of the disease. EFFECT: increases accuracy of the method. 2 dwg
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Authors
Dates
1994-08-30—Published
1991-02-07—Filed