FIELD: veterinary medicine. SUBSTANCE: method involves cultivation of virus-producing cells in growing medium containing Eagle's medium, PGMS, horse blood serum or serum of reindeer blood, and vitamin-salt additives (g/l): dihydrate monosodium phosphate 0.27-0.81, potassium chloride 0.045-0.135, heptahydrate magnesium sulfate 0.18-0.72, calcium chloride 0.00009-0.000207, sodium carbonate 0.0009-0.0036, glucose 0.18-0.63, thiamine chloride 0.00000018-0.00000072, riboflavin 0.00000018-0.00000072, calcium pantogenate 0.00000018-0.00000072, pyridoxin 0.00000018-0.00000072, folic acid 0.00000018-0.00000072, nicotine amide 0.00000018-0.00000072, isoinosite 0.00000018-0.00000072, L-glutamin 0.00008-0.0003, sodium chloride 2.79-3.15, cholin chloride 0.00000018-0.00000072, phenol red 0.00000018-0.00000072, dry hydrolyzate of muscle albumen 443.00-448.00, blood serum (of horse or reindeer) 85.0-95.0, Eagle's medium - the rest. EFFECT: more efficient method; higher product quality. 1 tbl
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Authors
Dates
1994-10-15—Published
1992-07-15—Filed