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Application

4736209/13, 1989-07-26

(22)

Actual filing date

1989-07-26

(45)

Published

1994-10-30

(72)

Inventor

Shirochina T.P.Filippova V.E.Kalunjants K.A.Kochetkova A.A.Shanenko E.F.

(73)

Holder

Moskovskaja Gosudarstvennaja Akademija Pishchevykh Proizvodstv

METHOD FOR PRODUCING PECTINESTERASE Russian patent published in 1994 - IPC

Abstract RU 2022013 C1

FIELD: biotechnology. SUBSTANCE: method involves treating the solution of complex pectolytic ferment preparation in cathode space of diaphragm electrolyzer at a current density of 0,05-30,00 mА/cm2 to pH 10.5-11.5, holding out, separation of sediment, bringing pH to 4.5-5.5 and extracting the target product. Solution of complex pectolytic ferment preparation is treated with aqueous solution of sodium salt of inorganic acid in a concentration of 0.3-2.0% . In above-mentioned pH interval the pectinesterase is stable and its yield is 56-67%. EFFECT: method ensures removal of alkali-labial proteins including polygalacturonasa with maximum preservation of pectinesterase in solution. 3 tbl

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RU 2 022 013 C1

Authors

Shirochina T.P.

Filippova V.E.

Kalunjants K.A.

Kochetkova A.A.

Shanenko E.F.

Dates

1994-10-30—Published

1989-07-26—Filed

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