FIELD: biotechnology, genetic engineering. SUBSTANCE: DNA fragment encoding polypeptide showing nitrile hydratase activity is prepared from the bacterial cells Pseudomonas chlororaphis B23. This nitrile hydratase shows capability to hydrate nitriles to amides. Recombinant DNA containing this gene and transformant formed by transformation of strain E. coli with recombinant DNA were prepared. Invention involves also a method of nitrile hydratase preparing using this transformant. EFFECT: preparing of DNA fragment indicated above. 5 cl, 10 dwg
| Title |
Year |
Author |
Number |
| DNA FRAGMENT ENCODING POLYPEPTIDE EXHIBITING ACTIVITY OF NITRILE HYDRATASE, RECOMBINANT PLASMID DNA ENCODING POLYPEPTIDE SHOWING NITRILE HYDRATASE PROPERTIES, STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF POLYPEPTIDE SHOWING NITRILE HYDRATASE PROPERTIES, METHOD OF PREPARING POLYPEPTIDE SHOWING NITRILE HYDRATASE PROPERTIES |
1991 |
- Terukhiko Beppu[Jp]
- Khideaki Jamada[Jp]
- Toru Nagasava[Jp]
- Suekharu Khorinouti[Jp]
- Makoto Nisijama[Jp]
|
RU2081173C1 |
| METHOD OF AMIDE PRODUCING |
1992 |
- Terukhiko Beppu[Jp]
- Khideaki Jamada[Jp]
- Toru Nagasava[Jp]
- Suekharu Khorinouti[Jp]
- Makoto Nisijama[Jp]
|
RU2082761C1 |
| DNA FRAGMENT ENCODING POLYPEPTIDE WITH ACTIVITY OF NITRILE HYDRATASE, RECOMBINANT PLASMID DNA PNHJ20L ENCODING POLYPEPTIDE, STRAIN ESCHERICHIA COLI TG1/PNHJ20L, METHOD OF PREPARING POLYPEPTIDE WITH NITRILE HYDRATASE PROPERTY |
1991 |
- Terukhiko Beppu
- Khideaki Jamada
- Toru Nagasava
- Suekharu Khorinouti
- Makoto Nisijama
|
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| METHOD FOR PREPARATION OF POLYPEPTIDE SHOWING ACTIVITY OF HUMAN TUMOR NECROSIS FACTOR |
0 |
- Mosaaki Yamada
- Yasudzhi Furutani
- Mitsie Notake
- Yuniti Yamagisi
|
SU1739855A3 |
| METHOD FOR PREPARATION OF POLYPEPTIDE WITH PROUROKINASE PROPERTIES AND A STRAIN OF BACTERIA ESCHERICHIA COLI - A PRODUCER OF POLYPEPTIDE WITH PROUROKINASE PROPERTIES (VARIANTS) |
0 |
- Tosio Miyake
- Yasuo Khibino
- Eiti Kobayasi
- Ken Vatabe
- Muneku Omori
- Tetsuzoi Miki
- Midori Ekoyama
- Rejko Matsumoto
- Kazuo Vatanabe
- Naganori Numao
|
SU1695827A3 |
| IMPROVED NITRILE HYDRATASE |
2015 |
- Kitakhara Aya
- Akiyama Takanori
- Vakamatsu Miki
- Vatanabe Fumiaki
|
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| METHOD FOR PREPARATION OF HYBRIDOUS PLASMINOGENE ACTIVATOR, CONTAINING REGION, RESPONSIBLE FOR AFFINITY WITH FIBRIN OF TISSUE PLASMINOGENE ACTIVATOR, AND REGION, RESPONSIBLE FOR ENZYME ACTIVITY OF PROUROKINASE POLYPERTIDE |
0 |
- Mitito Tagava
- Masakatsu Vada
- Masayuki Yamada
|
SU1732814A3 |
| METHOD OF PREPARING FACTOR STIMULATING GRANULOCYTE COLONY FORMATION |
1986 |
- Tatsumi Jamazaki[Jp]
- Sigekazu Nagata[Jp]
- Masajuki Tsutija[Jp]
- Juichi Khirata[Jp]
- Osami Jamamoto[Jp]
- Jasuo Sekimori[Jp]
|
RU2057809C1 |
| DNA SEQUENCE, PROTEIN, METHOD OF PROTEIN PRODUCING |
1991 |
- Kendzi Mitsusima[Jp]
- Akio Takimoto[Jp]
- Sigeo Jagi[Jp]
- Takajasu Sonojama[Jp]
|
RU2103364C1 |
| CYANIDE-RESISTANT NITRILE HYDRATASES |
2005 |
- Ossval'D Shteffen
- Vekkbekker Kristof
- Khutmakher Klaus
- Gerasimova Tat'Jana
- Novikov Andrej
- Rjabchenko Ljudmila
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|
RU2385876C2 |
