FIELD: microbiological industry, biotechnology. SUBSTANCE: cells of the strain E. coli were transformed with recombinant plasmid containing fragment of chromosomal DNA Pseudomonas chlororaphis B-23 which encodes enzyme nitrile hydratase. Then recombinant colonies were selected and cultured under conditions providing the production of enzyme protein. Then substrate (nitrile consisting of 2-4 carbon atoms) is added to suspension of cell-producers and mixture is incubated for time necessary for conversion the substrate to the corresponding amide. EFFECT: improved method of amide producing. 10 dwg
| Title |
Year |
Author |
Number |
| NUCLEIC ACID FRAGMENT ENCODING POLYPEPTIDE SHOWING NITRILE HYDRATASE ACTIVITY, RECOMBINANT PLASMID DNA PPCL 4 ENCODING POLYPEPTIDE SHOWING NITRILE HYDRATASE ACTIVITY, STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF POLYPEPTIDE SHOWING NITRILE HYDRATASE ACTIVITY, AND A METHOD OF NITRILE HYDRATASE PREPARING |
1991 |
- Terukhiko Beppu[Jp]
- Khideaki Jamada[Jp]
- Toru Nagasava[Jp]
- Suekharu Khorinouti[Jp]
- Makato Nisijama[Jp]
|
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1991 |
- Terukhiko Beppu[Jp]
- Khideaki Jamada[Jp]
- Toru Nagasava[Jp]
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- Makoto Nisijama[Jp]
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1991 |
- Terukhiko Beppu
- Khideaki Jamada
- Toru Nagasava
- Suekharu Khorinouti
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|
RU2173708C2 |
| DNA SEQUENCE, PROTEIN, METHOD OF PROTEIN PRODUCING |
1991 |
- Kendzi Mitsusima[Jp]
- Akio Takimoto[Jp]
- Sigeo Jagi[Jp]
- Takajasu Sonojama[Jp]
|
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| METHOD FOR PREPARATION OF POLYPEPTIDE WITH PROUROKINASE PROPERTIES AND A STRAIN OF BACTERIA ESCHERICHIA COLI - A PRODUCER OF POLYPEPTIDE WITH PROUROKINASE PROPERTIES (VARIANTS) |
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- Muneku Omori
- Tetsuzoi Miki
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- Kazuo Vatanabe
- Naganori Numao
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SU1695827A3 |
| METHOD FOR PREPARATION OF HYBRIDOUS PLASMINOGENE ACTIVATOR, CONTAINING REGION, RESPONSIBLE FOR AFFINITY WITH FIBRIN OF TISSUE PLASMINOGENE ACTIVATOR, AND REGION, RESPONSIBLE FOR ENZYME ACTIVITY OF PROUROKINASE POLYPERTIDE |
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- Mitito Tagava
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|
SU1732814A3 |
| RECOMBINANT TISSUE ACTIVATOR OF PLASMINOGEN AND METHOD FOR ITS PRODUCTION |
1988 |
- Jasusi Kavauti[Jp]
- Tosijuki Takemoto[Jp]
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- Yutaka Sasaki
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SU1367844A3 |
| SHUTTLE VECTOR PLASMID ABLE TO REPLICATION IN CELLS ESCHERICHIA COLI AND ANACYSTIS NIDULANS, SHUTTLE VECTOR PLASMID PBAX18 ABLE TO REPLICATION IN CELLS ESCHERICHIA COLI AND ANACYSTIS NIDULANS AND SHUTTLE VECTOR PLASMID PBAX20 ABLE TO REPLICATION IN CELLS ESCHERICHIA COLI AND ANACYSTIC NIDULANS |
1992 |
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| STRAIN OF YEAST PICHIA PASTORIS AS PRODUCER OF LEUKOCYTE HUMAN INTERFERON-16, RECOMBINANT PLASMID DNA PHIN AND METHOD FOR ITS CONSTRUCTION |
2002 |
- Padkina M.V.
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|
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