FIELD: molecular biology, genetic engineering. SUBSTANCE: invention describes amino acid and nucleotide sequences of α- and b-subunit of nitrile hydratase of H-type isolated from Rhodococcus rhodochrous J-1. DNA fragment encoding nitrile hydratase is inserted in expression vector and recombinant vector is used for E.coli transformation. Obtained strain produces nitrile hydratase at enhanced efficiency as compared with usually used microorganisms. EFFECT: enhanced effectiveness of nitrile hydratase expression. 4 cl, 9 dwg
| Title |
Year |
Author |
Number |
| DNA FRAGMENT ENCODING POLYPEPTIDE WITH ACTIVITY OF NITRILE HYDRATASE, RECOMBINANT PLASMID DNA PNHJ20L ENCODING POLYPEPTIDE, STRAIN ESCHERICHIA COLI TG1/PNHJ20L, METHOD OF PREPARING POLYPEPTIDE WITH NITRILE HYDRATASE PROPERTY |
1991 |
- Terukhiko Beppu
- Khideaki Jamada
- Toru Nagasava
- Suekharu Khorinouti
- Makoto Nisijama
|
RU2173708C2 |
| NUCLEIC ACID FRAGMENT ENCODING POLYPEPTIDE SHOWING NITRILE HYDRATASE ACTIVITY, RECOMBINANT PLASMID DNA PPCL 4 ENCODING POLYPEPTIDE SHOWING NITRILE HYDRATASE ACTIVITY, STRAIN OF BACTERIUM ESCHERICHIA COLI - A PRODUCER OF POLYPEPTIDE SHOWING NITRILE HYDRATASE ACTIVITY, AND A METHOD OF NITRILE HYDRATASE PREPARING |
1991 |
- Terukhiko Beppu[Jp]
- Khideaki Jamada[Jp]
- Toru Nagasava[Jp]
- Suekharu Khorinouti[Jp]
- Makato Nisijama[Jp]
|
RU2028380C1 |
| METHOD OF AMIDE PRODUCING |
1992 |
- Terukhiko Beppu[Jp]
- Khideaki Jamada[Jp]
- Toru Nagasava[Jp]
- Suekharu Khorinouti[Jp]
- Makoto Nisijama[Jp]
|
RU2082761C1 |
| IMPROVED NITRILE HYDRATASE |
2015 |
- Kitakhara Aya
- Akiyama Takanori
- Vakamatsu Miki
- Vatanabe Fumiaki
|
RU2689606C2 |
| METHOD FOR PREPARATION OF POLYPEPTIDE WITH PROUROKINASE PROPERTIES AND A STRAIN OF BACTERIA ESCHERICHIA COLI - A PRODUCER OF POLYPEPTIDE WITH PROUROKINASE PROPERTIES (VARIANTS) |
0 |
- Tosio Miyake
- Yasuo Khibino
- Eiti Kobayasi
- Ken Vatabe
- Muneku Omori
- Tetsuzoi Miki
- Midori Ekoyama
- Rejko Matsumoto
- Kazuo Vatanabe
- Naganori Numao
|
SU1695827A3 |
| DNA SEQUENCE, PROTEIN, METHOD OF PROTEIN PRODUCING |
1991 |
- Kendzi Mitsusima[Jp]
- Akio Takimoto[Jp]
- Sigeo Jagi[Jp]
- Takajasu Sonojama[Jp]
|
RU2103364C1 |
| NITRILE HYDRASE FROM Rhodococcus |
2005 |
- Osval'D Shteffen
- Ferzekk Shtefan
- Dajting Uta
- Vekkbekker Kristov
- Khutmakher Klaus
- Binder Mikhaehl'
- Kula Marija-Regina
- Odendal' Konrad
|
RU2385932C2 |
| METHOD FOR PREPARATION OF HYBRIDOUS PLASMINOGENE ACTIVATOR, CONTAINING REGION, RESPONSIBLE FOR AFFINITY WITH FIBRIN OF TISSUE PLASMINOGENE ACTIVATOR, AND REGION, RESPONSIBLE FOR ENZYME ACTIVITY OF PROUROKINASE POLYPERTIDE |
0 |
- Mitito Tagava
- Masakatsu Vada
- Masayuki Yamada
|
SU1732814A3 |
| CYANIDE-RESISTANT NITRILE HYDRATASES |
2005 |
- Ossval'D Shteffen
- Vekkbekker Kristof
- Khutmakher Klaus
- Gerasimova Tat'Jana
- Novikov Andrej
- Rjabchenko Ljudmila
- Janenko Aleksander
- Egorova Ksenija
|
RU2385876C2 |
| METHOD FOR PREPARATION OF POLYPEPTIDE SHOWING PROPERTIES OF TUMOR NECROSIS FACTOR |
0 |
- Yuniti Yamagisi
- Khitosi Kavasima
- Ryudzi Furuta
- Khirotada Kotani
- Katukhisa Nakata
|
SU1762761A3 |
