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2 031 656

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IPC

(21) (22)

Application

5049029/13, 1992-04-28

(22)

Actual filing date

1992-04-28

(45)

Published

1995-03-27

(72)

Inventor

Konarev A.A.Agadzhanova L.V.Pomogaeva L.S.Bezgin V.M.Shevyrev N.S.Nichveeva L.D.Solodov E.N.Kozlov V.E.

(73)

Holder

Kurskaja Biofabrika

METHOD FOR PREPARING TUBERCULIN Russian patent published in 1995 - IPC

Abstract RU 2031656 C1

FIELD: biotechnology, veterinary. SUBSTANCE: method involves cultivating of tuberculosis microbacteria, separation of culture liquid and isolating tuberculin of said liquid with the help of trichloracetic acid. Thus prepared tuberculin is reprecipitated by ammonia sulfate. The process is followed by desalting till final concentration of ammonia sulfate in tuberculin solution is 0.02-0.04 % and by sterilization of desired product. Mentioned above desalting is carried out with the help of electrodialysis at 15-20 C and pH 6-8. Linear rate of reaction mass is 0.015-0.023 m/s, the process being carried out at constant concentration of tuberculin in said solution. EFFECT: improves efficiency of the method. 1 tbl

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RU 2 031 656 C1

Authors

Konarev A.A.

Agadzhanova L.V.

Pomogaeva L.S.

Bezgin V.M.

Shevyrev N.S.

Nichveeva L.D.

Solodov E.N.

Kozlov V.E.

Dates

1995-03-27—Published

1992-04-28—Filed