FIELD: medicine; microbiology. SUBSTANCE: to obtain tuberculin from cultural filtrate, it is preconcentrated and followed by protein precipitation with trichloroacetic acid. Resulting acid concentration in the filtrate is 8-10% . Protein reprecipitation with ammonium sulfate is to be performed at neutral pH values. Heat treatment of the standard tuberculin solution under up to 80-90 C allows to stabilize the protein without using stabilizing additives ( such as Twin-80). EFFECT: steady activity level, high specificity rate. 2 cl, 2 tbl
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