FIELD: biological engineering; immunology. SUBSTANCE: method involves immobilization of antibodies and their treatment with marked antigen in the presence of the antigen to be determined. Antibody immobilization and their treatment is carried out in flow-through mode using fluorimetric cuvette with an optical path length being 0.005-0.1 mm. Variation coefficient of the results of analysis is equal to 10-15% EFFECT: enhanced accuracy in detecting the presence of antigens.
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