METHOD OF MULTIANALYTIC IMMUNE ASSAY WITH USING MICROPARTICLES Russian patent published in 2010 - IPC G01N33/58 

FIELD: medicine.

SUBSTANCE: invention refers to biology and medicine, namely to immunodiagnosis. There is offered method of multianalytic immune assay based on immunochemical, genetic and other types of reactions of biospecific binding analyte and ligands. There are mixed various categories of microparticles coated with biospecific reagents for binding of various required analytes and marked with one or more fluorochromes in various concentrations emitting a long-living fluorescence. The analysed sample and biospecific developing reagent marked with a detecting fluorochrome with a short-living fluorescence with its excitation area being outside that of fluorochromes with long-living fluorescence are added to the particle mixture. It is followed with reaction for biospecific complex formation. The prepared biospecific complexes are deposited on a solid-phase carrier. The fluorescence emission of all fluorochromes is excited with emitters in two spectral ranges herewith measuring an amount of long-living fluorescence in a time resolution mode to identify the microparticle and an amount of short-living fluorescence of detecting fluorochrome for measuring concentration of required analytes. Thus the concentration ratio of long fluorescing fluorochromes in microparticles for detecting the same type of analyte is constant, and for determining different types of analytes, the concentration ratio differs at least twice.

EFFECT: possibility of simultaneous analysis of great array of microparticles on a solid phase, ie increased multiplexity of the analysis, and wide-range measurement of concentration of required analytes.

6 cl; 3 ex, 10 dwg