FIELD: biotechnology, molecular biology. SUBSTANCE: copying and expresssion of nucleic acids is carried out in the medium containing cell-free enzymatic system and substrates which are necessary for exponential multiplication and/or expression of nucleic acids. Liquid phase is immobilized before incubation by placing into the organic or inorganic solid base showing porous, fibrous, netted, capillar, folded or laminated structure, average pore size is from 100 mcm to 5 nm. EFFECT: improved method of nucleic acid copying. 21 cl, 2 dwg
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Authors
Dates
1995-11-20—Published
1992-10-14—Filed