METHODS AND MEANS OF PRODUCING SEQUENCING LIBRARY Russian patent published in 2024 - IPC C12Q1/6806 

FIELD: biotechnology.

SUBSTANCE: described is a method for sequencing a nucleic acid, comprising: obtaining a sample containing a nucleic acid; bringing the sample into contact with a DNA-binding molecule containing a DNA dye; bringing the sample into contact with an insertion enzyme complex to obtain labeled nucleic acid fragments, wherein the insertion enzyme complex is inhibited by the DNA-binding molecule; and sequencing the labeled nucleic acid fragments to obtain sequence readings. Also described is a method of inhibiting, reducing or eliminating secondary sequencing readings, involving: obtaining a sample containing primary nucleic acids and secondary nucleic acids; bringing the sample into contact with a DNA-binding molecule, which preferentially binds secondary nucleic acids, where the DNA-binding molecule contains a DNA dye; and performing DNA transposition on open chromatin, wherein secondary nucleic acids are not transposed or are transposed with lower efficiency than primary nucleic acids. Also disclosed is a nucleic acid library for sequencing, obtained by said methods, comprising primary sequencing readings, obtained by DNA sequencing, wherein the nucleic acid library does not include or has a reduced representation of secondary sequencing readings due to DNA-binding molecules, which are preferentially bound to secondary DNA, where the DNA-binding molecule contains a DNA dye.

EFFECT: invention extends the range of sequencing methods.

26 cl, 5 dwg, 1 tbl, 2 ex