FIELD: veterinary virology. SUBSTANCE: vaccine virus strain "КБК" of infectious bursal avian disease is cultured and titrated on the transplantable cell line ВГМ-70. Strain is actively accumulated in cell cytoplasm ВГМ-70- after 36-48 hr at virus titer 107,5-108,0. Formaldehyde at concentration 0.1% for 24 hr inactivates virus. Strain is deposited in Collection ГВК at N 2292. Administration of living strain to chicken of different age with drinking water (dilution is 1:1000-1:1500), or intramuscular or subcutaneous injection to adult bird makes expressing immunity. Virus does not contain any impurities, it shows specificity and activity. EFFECT: enhanced quality of virus strain.
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Authors
Dates
1996-02-10—Published
1993-06-01—Filed