Show metadata Hide metadata

(19)

(11)

2 265 667

(13)

(51)

IPC

(21) (22)

Application

2003138290/13, 2003-12-24

(24)

Start date

2003-12-24

(22)

Actual filing date

2003-12-24

(45)

Published

2005-12-10

(72)

Inventor

Glotov A.G.Oreshkova S.F.Zhirakovskaja E.V.Glotova T.I.Nekrasova N.V.Nefedchenko A.V.

(73)

Holder

Gosudarstvennoe Nauchnoe Uchrezhdenie Institut Ehksperimental'Noj Veterinarii Sibiri I Dal'Nego Vostoka Sibirskogo Otdelenija Rossel'Khozakademii Iehvsidv So Raskhn)

METHOD FOR DETECTING VIRUS IRT KRS IN POLYMERASE CHAIN REACTION AND FOLLOWING DIFFERENTIATION OF VACCINE STRAIN TK-A FROM EPIZOOTIC STRAINS AND ISOLATES BY PLRF-ANALYSIS Russian patent published in 2005 - IPC

Abstract RU 2265667 C2

FIELD: biotechnology, genetic engineering, virology.

SUBSTANCE: invention proposes a method for isolating virus IRT KRS in polymerase chain reaction. Method involves carrying out polymerase chain reaction with primers B1 and B2 followed by differentiation of detected DNA in case of positive result in PCR reaction. Result is considered to be positive if PCR product corresponds to size of fragment consisting of 464 pair bases. For differentiation PCR product is treated with (restriction) endonuclease Sac II. As result, the presence of one fragment with length 464 pair bases corresponds to the vaccine strain TK-A and two fragments of size 343 and 121 pair bases correspond to epizootic strains and isolates.

EFFECT: improved detecting method and analysis.

1 dwg, 3 tbl, 6 ex

Similar patents RU2265667C2

Title	Year	Author	Number
METHOD FOR INDICATION AND DIFFERENTIATION OF VACCINE STRAINS AND FIELD ISOLATES OF CLASSIC HOG CHOLERA VIRUS	2001	Bezborodova S.V. Andreev V.G. Gusev A.A.	RU2196992C2
SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND METHOD FOR DETECTING CATTLE INFECTIOUS RHINOTRACHEITIS VIRUS DNA USING SPECIFIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION (PCR)	2003	Glotov A.G. Koteneva S.V. Glotova T.I. Nekrasova N.V. Shuljak A.F.	RU2259398C1
METHOD FOR DIFFERENTIATION OF LAVR VACCINE STRAIN OF INFECTIOUS RHINOTRACHEITIS AGENT FROM OTHER STRAINS AND FIELD ISOLATES OF FELINE INFECTIOUS RHINOTRACHEITIS VIRUS BY ANALYSING MELTING TEMPERATURE CURVE OF PCR V1-GENE PRODUCTS USING ORANGE POLYMER DYE SUPERNOVA V605	2024	Doronin Maksim Igorevich Galkina Tatiana Sergeevna Mikhalishin Dmitrii Valerevich Guseva Marina Nikolaevna Okovytaia Tatiana Vladimirovna Elkina Iuliia Sergeevna Budina Olesia Olegovna Gochmuradov Ykhlas Muradovich Koroleva Kseniia Valerevna	RU2824661C1
METHOD AND SET FOR DETECTION OF AFRICAN PLAGUE PIG VIRUS DNA BY METHOD OF POLYMERASE CHAIN REACTION	1996	Tsybanova L.Ja. Vishnjakov I.F. Tsybanov S.Zh.	RU2125089C1
SYNTHETIC OLIGONUCLEOTIDE-PRIMERS USED FOR DETECTION OF DNA OF CATTLE INFECTIOUS RHINOTRACHEITIS AND PUSTULAR VULVOVAGINITIS HERPES VIRUS TYPE 1	2002	Susloparov M.A. Noskova N.V. Susloparov I.M. Glotov A.G. Koteneva S.V.	RU2241751C2
SYNTHETIC OLIGONUCLEOTIDE PRIMERS, METHOD FOR DETECTING AND DIFFERENTIATING RIFT VALLEY VIRUS STRAINS BY POLYMERASE CHAIN REACTION AND RESTRICTION ENZYME DIGEST ANALYSIS	2010	Vlasova Natal'Ja Nikiforovna Zakutskij Nikolaj Ivanovich Tsybanov Sodnom Zham'Janovich Khukhorova Irina Jur'Evna Kapustina Ol'Ga Vladimirovna	RU2457255C2
METHOD OF DETECTION AND DIFFERENTIATION OF ENTEROVIRUS RNA	2000	Golitsina L.N. Novikova N.A.	RU2189396C2
OLIGONUCLEOTIDE PRIMERS AND FLUORESCENCE-LABELED PROBES, A METHOD AND A TEST SYSTEM FOR DIFFERENTIATING THE GENOME OF THE VACCINE STRAIN AND FIELD ISOLATE OF THE VIRAL INFECTIOUS CERMET DERMATITIS WITH ADDITIONAL DETECTION OF THE CAPRIPOX VIRUS GENOME BY REAL-TIME POLYMERASE CHAIN REACTION	2018	Sprygin Aleksandr Vladimirovich Kononov Aleksandr Vladimirovich Byadovskaya Olga Petrovna Pestova Yana Evgenevna Kostrova Evgeniya Sergeevna Nesterov Aleksandr Aleksandrovich Prutnikov Pavel Vladimirovich Kononova Svetlana Vladimirovna	RU2699195C1
SET OF SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF RNA OF RABIES VIRUS AND METHOD OF IDENTIFICATION OF RNA OF RABIES VIRUS BY MEANS OF SYNTHETIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION WITH REVERSE TRANSCRIPTION (RT-PCR)	2014	Ivanov Arkadij Vasil'Evich Khismatullina Nailja Anvarovna Usol'Tsev Konstantin Valer'Evich Guljukin Aleksej Mikhajlovich Aleksandrova Natal'Ja Mikhajlovna Juzhakov Anton Gennad'Evich Sabirova Valentina Vasil'Evna Chernov Al'Bert Nikolaevich Ivanov Aleksandr Arkad'Evich Zaberezhnyj Aleksej Dmitrievich Samerkhanov Il'Nur Irshatovich Parshikova Anna Vladimirovna Faizov Tagir Khadievich	RU2575088C1
METHOD FOR DIFFERENTIATION OF VACCINE STRAIN "VGNKI" OF ADENOVIRAL INFECTION CAUSATIVE AGENT FROM OTHER STRAINS AND FIELD ISOLATES BY ANALYZING MAXIMUM MELTING POINTS OF PCR PRODUCTS USING SUPERNOVA v605 TANDEM FLUORESCENT DYE	2023	Doronin Maksim Igorevich Malygtn Maksim Pavlovich Galkina Tatiana Sergeevna Mikhalishin Dmitrii Valerevich Kononov Aleksandr Vladimirovich Guseva Marina Nikolaevna Prokopenko Marina Anatolevna	RU2825893C1

RU 2 265 667 C2

Authors

Glotov A.G.

Oreshkova S.F.

Zhirakovskaja E.V.

Glotova T.I.

Nekrasova N.V.

Nefedchenko A.V.

Dates

2005-12-10—Published

2003-12-24—Filed