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2 158 306

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IPC

(21) (22)

Application

99101603/13, 1999-01-19

(24)

Start date

1999-01-19

(22)

Actual filing date

1999-01-19

(45)

Published

2000-10-27

(72)

Inventor

Semenikhin V.I.Donchenko A.S.Oreshkova S.F.Chekishev V.M.Jurik S.A.Il'Ichev A.A.

(73)

Holder

Institut Ehksperimental'Noj Veterinarii Sibiri I Dal'Nego Vostoka So RaskhnGosudarstvennyj Nauchnyj Tsentr Virusologii I Biotekhnologii

METHOD OF DETECTION OF CATTLE VIRAL DIARRHEA VIRUS (MUCOSA DISEASE) USING SPECIFIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION Russian patent published in 2000 - IPC

Abstract RU 2158306 C2

FIELD: molecular biology, veterinary science. SUBSTANCE: invention relates to method that can be used for detection of cattle viral diarrhea. Method involves synthesis of oligonucleotide primers 5'-TAG-ATT-CCA-GAC-CGA-CCC-3' and 5'-GGA-AGG-GAT-ACA-ACG-GGC-3' and synthesis of complementary DNA on DNA matrix. Then amplification of synthesized DNA is carried out by method of polymerase chain reaction and polymerase chain reaction products size is analyzed. Proposed method ensures to differentiate cattle viral diarrhea virus from the close-related classical porcine plague virus. EFFECT: improved method of virus detection. 1 tbl, 1 ex

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RU 2 158 306 C2

Authors

Semenikhin V.I.

Donchenko A.S.

Oreshkova S.F.

Chekishev V.M.

Jurik S.A.

Il'Ichev A.A.

Dates

2000-10-27—Published

1999-01-19—Filed