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2 120 994

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(51)

IPC

(21) (22)

Application

97103934/13, 1997-03-12

(22)

Actual filing date

1997-03-12

(45)

Published

1998-10-27

(72)

Inventor

Semenikhin V.I.Puzyrev A.T.Donchenko A.S.Oreshkova S.F.Chekishev V.M.Il'Ichev A.A.Gerb P.E.

(73)

Holder

Institut Ehksperimental'Noj Veterinarii Sibiri I Dal'Nego Vostoka So Ran

METHOD OF DETECTION OF PIG CLASSIC PLAGUE VIRUS Russian patent published in 1998 - IPC

Abstract RU 2120994 C1

FIELD: molecular biology, genetic engineering. SUBSTANCE: method involves synthesis of oligonucleotide primers 5'-CTGCTGGCCTGG- -GTGACTACG-3' and 5'-ATAACTCAATGGAACCTG-3' and synthesis of complementary DNA on RNA-template. Then amplification of synthesized DNA is carried out by method of polymerase chain reaction and size products of polymerase chain reaction is analyzed. Method ensures to differentiate pig classic plague virus from closely related cattle diarrhea virus. EFFECT: high specificity of method. 1 tbl, 1 ex

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RU 2 120 994 C1

Authors

Semenikhin V.I.

Puzyrev A.T.

Donchenko A.S.

Oreshkova S.F.

Chekishev V.M.

Il'Ichev A.A.

Gerb P.E.

Dates

1998-10-27—Published

1997-03-12—Filed