FIELD: biotechnology, genetic engineering, molecular biology, veterinary science.
SUBSTANCE: invention proposes synthetic primers that are complementary to highly conservative region in cattle infectious rhinotracheitis virus genome in region of thymidine kinase (tk) gene. Proposed primers are used for detecting cattle infectious rhinotracheitis virus DNA using polymerase chain (PCR) reaction method. Proposed method involves carrying out amplification by PCR method. For carrying out PCR method involves using synthetic primers that are complementary to highly conservative region in cattle infectious rhinotracheitis virus genome in region of thymidine kinase gene (tk). Results of the reaction are evaluated without preliminary restriction of the prepared product. Result is considered to be positive if PCR product corresponds to fragment size 298 base pairs. Method can be used in veterinary virology for detection of infectious diseases in agricultural animals, in particular, for detection of infectious rhinotracheitis in cattle.
EFFECT: improved detecting method.
2 tbl, 7 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETECTING VIRUS IRT KRS IN POLYMERASE CHAIN REACTION AND FOLLOWING DIFFERENTIATION OF VACCINE STRAIN TK-A FROM EPIZOOTIC STRAINS AND ISOLATES BY PLRF-ANALYSIS | 2003 |
|
RU2265667C2 |
| SYNTHETIC OLIGONUCLEOTIDE-PRIMERS USED FOR DETECTION OF DNA OF CATTLE INFECTIOUS RHINOTRACHEITIS AND PUSTULAR VULVOVAGINITIS HERPES VIRUS TYPE 1 | 2002 |
|
RU2241751C2 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND METHOD OF EXPOSING RNA VIRUS OF MUCOSAL DISEASE IN MUCUS OF CATTLE USING SPECIFIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION WITH SIMULTANEOUS DIFFERENTIATION OF VIRUS STRAINS TO 1 AND 2 GENOTYPES | 2007 |
|
RU2347812C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND METHOD OF BOVINE RESPIRATORY SYNCYTIAL INFECTION RNA VIRUS DETECTION BY SYNTHETIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION (PCR) | 2009 |
|
RU2405039C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR DETECTING 1A AND 1B VIRUS SUBGENOTYPES OF VIRAL DIARRHOEA - BOVINE MUCOSAL DISEASE AND METHOD OF APPLYING THEREOF | 2009 |
|
RU2409673C1 |
| METHOD OF DETECTION OF CATTLE VIRAL DIARRHEA VIRUS (MUCOSA DISEASE) USING SPECIFIC OLIGONUCLEOTIDE PRIMERS IN POLYMERASE CHAIN REACTION | 1999 |
|
RU2158306C2 |
| TEST SYSTEM FOR DETECTING DNA OF RHINOTRACHEITIS VIRUS (BOVINE HERPES VIRUS 1, BOHV-1) IN CATTLE | 2018 |
|
RU2700254C1 |
| METHOD AND SET FOR DETECTION OF AFRICAN PLAGUE PIG VIRUS DNA BY METHOD OF POLYMERASE CHAIN REACTION | 1996 |
|
RU2125089C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS AND METHOD FOR DETECTING RNA ATYPICAL PESTIVIRUS OF CATTLE | 2016 |
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| OLIGONUCLEOTIDE PRIMERS FOR DETECTING BOVINE ENZOOTIC LEUKAEMIA VIRUS BY POLYMERASE CHAIN REACTION | 2024 |
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RU2824666C1 |
