FIELD: genetic engineering, medicine. SUBSTANCE: immunoglobulin G binding the protein G is prepared by cloning gene G-protein from streptococcus G148 in E. coli. Protein is isolated from E. coli cells and purified by affinitive chromatography followed by separation of homogeneous protein. Protein shows high yield and binding constants and can be used in immunology, immunochemistry and medicinal diagnosis. EFFECT: increased yield, homogeneity and high affinity of G-protein. 8 dwg, 2 tbl
Title |
Year |
Author |
Number |
RECOMBINANT HSA-RECEPTOR OF STREPTOCOCCUS GROUP G |
1994 |
- Gupalova Tat'Jana Vital'Evna
- Orlova Svetlana Nikolaevna
- Totoljan Artem Akopovich
|
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RECOMBINANT DNA OF pG4223 AND PLASMID DNA PQE 30-pG4223, STRAIN OF ESCHERICHIA COLI M 15-G4223 WHICH ENABLE TO OBTAIN G4223 POLYPEPTIDE SELECTIVELY BINDING IgG, AND USE THEREOF IN AFFINE CHROMATOGRAPHY TO ISOLATE IgG |
2017 |
- Gupalova Tatyana Vitalievna
- Bormotova Elena Alekseevna
|
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RECOMBINANT POLYPEPTIDE A2 SELECTIVELY BINDING HSA, RECOMBINANT DNA pa2 CODING HSA-BINDING PART OF POLYPEPTIDE A2, PRODUCER THEREOF - RECOMBINANT STRAIN OF Escherichia coli M15-A2, CONTAINING RECOMBINANT PLASMID DNA pQE 32-pa2, FACILITATING PRODUCTION OF POLYPEPTIDE A2 AND USE OF POLYPEPTIDE A2 FOR DIAGNOSIS OF MICROALBUMINURIA AND SEPARATING HSA FROM BLOOD SERUM |
2011 |
- Gupalova Tat'Jana Vitalievna
- Bormotova Elena Alekseevna
- Totoljan Artem Akopovich
|
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RECOMBINANT GM PROTEIN CAPABLE OF BINDING Α2-MACROGLOBULIN AND APPLICATION THEREOF AS A LIGAND IN AFFINITY CHROMATOGRAPHY FOR EXTRACTING Α2-MACROGLOBULIN FROM HUMAN BLOOD SERUM |
2019 |
- Gupalova Tatyana Vitalievna
- Bormotova Elena Alekseevna
|
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RECOMBINANT DNA pA4, RECOMBINANT DNA pQE 30-pA4, PROVIDING OBTAINING POLYPEPTIDE A4, Esherichia coli STRAIN M 15-A4, TRANSFORMED WITH RECOMBINANT PLASMID DNA pQE 30-pA4, AND EXPRESSING RECOMBINANT POLYPEPTIDE A4, RECOMBINANT POLYPEPTIDE A4, POSSESSING ABILITY TO SELECTIVELY BIND HSA, AFFINE SORBENTS (VERSIONS) AND METHODS FOR HSA AND IgG REMOVAL FROM BLOOD SERUM (VERSIONS) |
2014 |
- Gupalova Tat'Jana Vitalievna
- Bormotova Elena Alekseevna
|
RU2572343C2 |
RECOMBINANT DNA pA3, RECOMBINANT DNA pQE 30-pA3 PROVIDING PRODUCING POLYPEPTIDE A3, STRAIN E. coli M 15-A3 TRANSFORMED BY RECOMBINANT PLASMID DNA pQE 30-pA3 AND EXPRESSING RECOMBINANT POLYPEPTIDE A3, RECOMBINANT POLYPEPTIDE A3 POSSESSING ABILITY TO BIND HUMAN SERUM ALBUMIN, AND RFA TEST SYSTEM FOR QUALITATIVE DETECTION OF MICROALBUMINURIA, TEST SYSTEM FOR QUANTITATIVE DETERMINATION OF MICROALBUMINURIA |
2013 |
- Gupalova Tat'Jana Vitalievna
- Bormotova Elena Alekseevna
|
RU2550255C2 |
METHOD FOR INTROGRESSION OF PATHOGENIC STREPTOCOCCUS GENES IN CHROMOSOMAL DNA OF PROBIOTIC STRAIN OF ENTEROCOCCUS FAECIUM L3 FOR EXPRESSION IN PILES |
2015 |
- Suvorov Aleksandr Nikolaevich
- Gupalova Tatyana Vitalievna
- Bormotova Elena Alekseevna
- Leonteva Galina Fedorovna
- Kramskaya Tatyana Anatolevna
- Grabovskaya Korneliya Borisovna
|
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RECOMBINANT PLASMID DNA pcL37 ENCODING POLYPEPTIDE WITH PROPERTIES OF HUMAN LIGHT CHAIN OF ANTIBODY AGAINST SMALLPOX VIRUS VACCINE, RECOMBINANT PLASMID DNA pcH37 ENCODING POLYPEPTIDE WITH PROPERTIES OF HEAVY CHAIN OF INDICATED ANTIBODY AND THEIR USING |
2006 |
- Tikunova Nina Viktorovna
- Shingarova Ljudmila Nikolaevna
- Jun Tat'Jana Ehverestovna
- Morozova Vera Vital'Evna
- Aliev Tejmur Kantamirovich
- Boldyreva Elena Filippovna
- Nekrasova Oksana Vasil'Evna
- Polykhalova Ilona Vladimirovna
- Panina Anna Alekseevna
- Kirpichnikov Mikhail Petrovich
- Il'Ichev Aleksandr Alekseevich
- Sandakhchiev Lev Stepanovich
|
RU2317330C2 |
RECOMBINANT DNA PROVIDING PRODUCTION OF POLYPEPTIDES P6, P7, P8 EXHIBITING PROTECTIVE PROPERTIES AGAINST STREPTOCOCCUS AGALACTIAE AND SELECTIVELY CONNECTING IgA |
2008 |
- Suvorov Aleksandr Nikolaevich
- Ustinovich Irina Anatol'Evna
- Meringova Ljudmila Fedorovna
- Leont'Eva Galina Fedorovna
- Grabovskaja Kornelija Borisovna
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|
RU2387715C2 |
RECOMBINANT POLYPEPTIDE StV POSSESSING PROTECTIVE PROPERTIES IN RELATION TO Streptococcus agalactiae, RECOMBINANT DNA StV, ITS CODING AND RECOMBINANT STRAIN Escherichia coli M15-StV CONTAINING RECOMBINANT PLASMID PQE-StV |
2012 |
- Suvorov Aleksandr Nikolaevich
- Gupalova Tat'Jana Vital'Evna
- Kuleshevich Evgenija Vladimirovna
- Grabovskaja Kornelija Borisovna
- Leont'Eva Galina Fedorovna
|
RU2542483C2 |