RECOMBINANT PLASMID DNA pcL37 ENCODING POLYPEPTIDE WITH PROPERTIES OF HUMAN LIGHT CHAIN OF ANTIBODY AGAINST SMALLPOX VIRUS VACCINE, RECOMBINANT PLASMID DNA pcH37 ENCODING POLYPEPTIDE WITH PROPERTIES OF HEAVY CHAIN OF INDICATED ANTIBODY AND THEIR USING Russian patent published in 2008 - IPC C12N15/79 C12N15/13 C07K16/08 C07K16/46 

FIELD: biotechnology, genetic engineering.

SUBSTANCE: invention describes recombinant plasmid DNAs constructed in vitro that comprise artificial genes for light and heavy chains of full-scale human antibody prepared by genetic engineering methods. These genes are created on basis of variable fragments of light and heavy chains of recombinant antibody 1F4 and constant human genes IgG1, cytomegalovirus promoter and polyadenylation site BGH. Plasmids provide biosynthesis of recombinant full-scale human antibodies of class IgG1 in mammalian cells. These antibodies interact specifically with smallpox vaccine virus. The affinity constant for prepared recombinant antibodies is 3.54 x 10⁹ ± 0.38 x 10⁹ M^-1. Plasmids are used by combined transfection of human cells HEK 293T. Prepared full-scale recombinant antibody against protein of size 27 kDa of smallpox virus vaccine can be used as a base for creature of pharmaceutical preparations used for diagnosis of some post-vaccine complications caused by smallpox virus vaccine. Also, preparations will comprise decreased therapeutic doses of immunoglobulins that will provide minimal undesirable immune response in patients after administration of the preparation.

EFFECT: valuable medicinal properties of plasmid DNA.

4 cl, 7 dwg, 6 ex