METHOD OF DRIED PROBIOTIC PREPARATION MAKING Russian patent published in 1996 - IPC

FIELD: medicine, veterinary. SUBSTANCE: strain of enterobacterium, for example, Bifidobacterium globosum, "БФ"-4 or Streptococcus faecium "ВГНКИ"-27 is grown on liquid nutrient medium based on casein hydrolysate for 12-15 hr. Native cultural fluid is cooled to 15-20 C and concentrated by 10-15-fold. Obtained concentrated biomass is washed off with 2.5-3.5% sucrose or lactose solution and dehydrated at (-25)-(-35) C. Concentration of native cultural fluid is carried out by ultrafiltration method on hollow fibers or by mictofiltration on flat diaphragms, or by separation at semiperiodic working regime. EFFECT: improved method of probiotic preparation making. 4 cl, 3 tbl