FIELD: biotechnology. SUBSTANCE: method involves raw grinding, extraction in acid medium, extract treatment, chromatography on sulfocationite, eluate treatment from cationite with hydrochloric acid up to pH = 1-3, precipitate separation, supernatant treatment with trichloroacetic acid up to its concentration 2.5-7.0%, inert proteins removing, desalting the end product and its purification by chromatography, precipitation and lyophilization of preparation. EFFECT: increased specific activity and purity of proteinase inhibitor.
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Authors
Dates
1996-10-20—Published
1993-03-19—Filed