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2 068 267

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IPC

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Application

5067036/14, 1992-09-01

(22)

Actual filing date

1992-09-01

(45)

Published

1996-10-27

(72)

Inventor

Vakhitov R.Z.Jusupov V.G.Nigamov F.N.Bojko N.A.Vakhitov R.Sh.Kozyreva M.Kh.

(73)

Holder

Nauchno-Proizvodstvennoe Ob"Edinenie

METHOD OF HYALURONIDASE PREPARING Russian patent published in 1996 - IPC

Abstract RU 2068267 C1

FIELD: biotechnology, medicinal preparations. SUBSTANCE: method involves milling bovine testicles and the following extraction of prepared homogenate with 0.1N acetic acid solution. Then extract is separated on filtering fabric and concentrated by ultrafiltration at tangential flow on diaphragms at retention threshold 5-15 kDa. Concentrate is subjected for two-fold freezing-thawing, hemopigment and high-molecular components were removed. Yield of the end product is increased by two-fold as compared with prototype. Method is used for medicinal preparation from animal raw. EFFECT: increased yield, simplified method. 2 tbl

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RU 2 068 267 C1

Authors

Vakhitov R.Z.

Jusupov V.G.

Nigamov F.N.

Bojko N.A.

Vakhitov R.Sh.

Kozyreva M.Kh.

Dates

1996-10-27—Published

1992-09-01—Filed

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