METHOD OF PREPARING STREPTOMYCETES STRAINS - PRODUCERS OF AVERMECTIN Russian patent published in 1997 - IPC

FIELD: microbiology. SUBSTANCE: method involves irradiation of spores of culture Streptomyces avermitilis by at least a single dose of braking short-impulse roentgen radiation at quantum energy 80-160 KeV, at impulse irradiation time 0.5-10^-7 s, and pauses between irradiation 5-25 s. Exposition dose for spore irradiation is 100-(2 x 10³) R. Then spores were kept at 5-7 C for 2-10 h and irradiated by at least a single dose of energetic electrons at energy value 80-200 KeV, at current density 6,10^-5÷5,0 A/cm², impulse irradiation time is 0.5-10^-7 s, and pauses between irradiation 5-25 s. Dose of electron irradiation is 10^-3-1.0 Megarad. EFFECT: improved method of highly active strains preparing.