FIELD: medicine. SUBSTANCE: method involves homogenization of animal spinal cord, extraction of biologically active substance with sodium chloride solution, centrifugation of the brain aqueous suspension, precipitation of substance with ammonium sulfate, precipitate dissolving in sodium chloride solution, desalting, chromatography and lyophilization. EFFECT: simplified method, high purity degree of biologically active substance, safety.
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Authors
Dates
1997-03-10—Published
1993-06-08—Filed