FIELD: biology, medicine, immunology.
SUBSTANCE: invention relates to a method for preparing antiserum against mammal CNSS peptides. Method is carried out by immunization of mammals (man, cat, pig) with CNSS peptides in combination with full Freund's adjuvant by subcutaneous and subconjunctive administration and by intravenous administration as a pure form. Antigen is administrated to the following mammals: rabbit, guinea pig and ass for three steps with break for 2 days between each step. Two days after the last administration of antigen antiserum against vertebrate CNSS peptides with a titer value 1:256 is prepared from heart of live fixed animal and stored it in native or lyophilized state at 4-5°C. Method provides preparing antiserum with high content of specific antibodies for short time, method shows low difficulty and economy and sterile serum can be stored for up to 2-3 years without loss of its activity.
EFFECT: improved preparing method.
3 ex
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