FIELD: biotechnology, genetic engineering, molecular biology. SUBSTANCE: method involves the construction of recombinant plasmid DNA containing the structural gene encoding polypeptide under control of promoter. Promoter can be viral or metallothionine one. Myeloma strain is transformed with the obtained recombinant DNA. Cells were cultured on the nutrient medium and the end polypeptide is isolated and purified. EFFECT: improved method of polypeptide preparing. 3 dwg , 2 tbl
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